Institute of Physics Ministry of Science and Education Republic of Azerbaijan

EFFECT OF RHODAMINE 6G LASER DYE ON PROTEIN AGGREGATION IN SILK FIBROIN FILMS: STRUCTURAL ANALYSIS BY SEM

A.J. Mammadova (Guliyeva)

ABSTRACT

Morphological changes occurring in Silk Fibroin -Rhodamine 6G films were examined using scanning electron microscopy (SEM). SEM analysis revealed that treatment of SF films with 70% methanol induces the formation of large aggregates. However, in the presence of RHD 6G, these protein aggregates appear to be fragmented into significantly smaller structures. X-ray diffraction (XRD) and Fourier-transform infrared (FTIR) spectroscopy further indicate that prolonged exposure of SF films to 70% methanol results in dehydration, leading to the dense packing of SF amyloids.

Keywords: silk fibroin, amyloid, aggregation, neurodegenerative diseases, fluorescence, SEM, XRD
DOI:10.70784/azip.2.2025251

Received: 21.05.2025
Internet publishing: 17.06.2025 AJP Fizika A 2025 02 az p.51-55

AUTHORS & AFFILIATIONS

Institute of Biophysics, Ministry of Science and Education Republic of Azerbaijan 117 Z. Khalilov, Baku, Azerbaijan
E-mail: a.k134@hotmail.com

Graphics and Images

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